LS-C185927

antibody from LSBio

Targeting: IPO13 IMP13, KIAA0724, RANBP13

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C185927

Provider

LSBio

Product name

IPO13 / LGL2 Antibody (aa901-963) LS-C185927

Antibody type

Polyclonal

Description

Antigen-specific affinity chromatography

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Storage

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Western blot

Enhanced validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of IPO13 expression in transfected 293T cell line by IPO13 polyclonal antibody. A: Non-transfected lysate., B: IPO13 transfected lysate. 7.5% SDS PAGE. IPO13 / LGL2 antibody diluted at 1:500

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunofluorescence of paraformaldehyde-fixed HeLa using Importin 13 antibody at 1:200 dilution.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IPO13 was immunoprecipitated from 1 mg of HeLa whole cell lysate using 10 ug of anti-IPO13 antibody . The precipitated IPO13 was detected by IPO13 / LGL2 antibody diluted at 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IPO13 was immunoprecipitated from 1 mg of HeLa whole cell lysate using 10 ug of anti-IPO13 antibody . The precipitated IPO13 was detected by IPO13 / LGL2 antibody diluted at 1:1000.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IPO13 was immunoprecipitated from 1 mg of HeLa whole cell lysate using 10 ug of anti-IPO13 antibody . The precipitated IPO13 was detected by IPO13 / LGL2 antibody diluted at 1:1000.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded CL1-5 xenograft using Importin 13 antibody at 1:100 dilution.