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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- PA5-87583 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2O Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: LVNEPYYNEA GFDSDRGLQE GYENSRCYNE MALIRVVQSM TQLVRRPPEV FEQEIRQHFS TGGWRLVNRI ESWLETHALL EKAQALPNGV PKASSSPEPP AVAELSDSGQ QEPEDGGPAP GEASQGSDSE GGAQGLASAS RDHTDQTSET APDASVPPSV KPKKRRKSYR SFLPEKSGYP DIGFPLFPLS KGFIKSIRGV LTQFRAALLE AGMPECTEDK; Positive Samples: A-431, HepG2, Jurkat, Mouse heart, Mouse brain; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.05 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O Polyclonal antibody (Product # PA5-87583) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UBE2O in extracts from normal (control) and UBE2O knockout (KO) HeLa cells using UBE2O Polyclonal Antibody (Product # PA5-87583) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of UBE2O in extracts of various cell lines using UBE2O Polyclonal Antibody (Product # PA5-87583) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of UBE2O was performed in HeLa cells using UBE2O Polyclonal Antibody (Product # PA5-87583).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of UBE2O was performed in 200 µg extracts of A-431 cells using UBE2O Polyclonal Antibody (Product # PA5-87583). Western blot was performed from the immunoprecipitate using UBE2O Polyclonal Antibody.
