Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP1-03336 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-03336, RRID:AB_1522642
- Product name
- Rabbit Polyclonal UBE2O Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Evidence for ubiquitin-regulated nuclear and subnuclear trafficking among Paramyxovirinae matrix proteins.
UBE2O negatively regulates TRAF6-mediated NF-κB activation by inhibiting TRAF6 polyubiquitination.
Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6.
Pentecost M, Vashisht AA, Lester T, Voros T, Beaty SM, Park A, Wang YE, Yun TE, Freiberg AN, Wohlschlegel JA, Lee B
PLoS pathogens 2015 Mar;11(3):e1004739
PLoS pathogens 2015 Mar;11(3):e1004739
UBE2O negatively regulates TRAF6-mediated NF-κB activation by inhibiting TRAF6 polyubiquitination.
Zhang X, Zhang J, Zhang L, van Dam H, ten Dijke P
Cell research 2013 Mar;23(3):366-77
Cell research 2013 Mar;23(3):366-77
Fine-tuning BMP7 signalling in adipogenesis by UBE2O/E2-230K-mediated monoubiquitination of SMAD6.
Zhang X, Zhang J, Bauer A, Zhang L, Selinger DW, Lu CX, Ten Dijke P
The EMBO journal 2013 Apr 3;32(7):996-1007
The EMBO journal 2013 Apr 3;32(7):996-1007
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2O Antibody [NBP1-03336] - Detection of Human UBE2O bon HeLa whole cell lysate using NBP1-03336.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2O Antibody [NBP1-03336] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-UBE2O antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: UBE2O Antibody [NBP1-03336] - Whole cell lysate (50 ug) from NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit antiUBE2O antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: UBE2O Antibody [NBP1-03336] - Detection of human UBE2O by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-UBE2O antibody A301-873A (lot A301-873A-3) used for IP at 3 ug per reaction. UBE2O was also immunoprecipitated by rabbit anti-UBE2O antibody BL7369. For blotting immunoprecipitated UBE2O, A301-873A was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.