PA5-120450

antibody from Invitrogen Antibodies

Targeting: SUPT6H KIAA0162, SPT6H

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

PA5-120450

Provider

Invitrogen Antibodies

Product name

SPT6 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive test controls include: U-87MG, Jurkat, HeLa, Mouse brain, Mouse heart, Mouse liver. The target is usually found in the following locations: Nucleus.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

3.74 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPT6 in Mouse heart using a SPT6 Polyclonal antibody (Product # PA5-120450) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SPT6 in various cell lines using a SPT6 Polyclonal antibody (Product # PA5-120450) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 10s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of SPT6 in U-2 OS cells using a SPT6 Polyclonal antibody (Product # PA5-120450). Blue: DAPI for nuclear staining.