Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- A700-065 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD96 Recombinant Rabbit Monoclonal Antibody (BLR065G)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- BLR065G
- Vial size
- 100 µL
- Concentration
- 1000 µg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD96 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, MOLT-4, HEK293T, A2058, Jurkat, CCRF-CEM, Hep-G2, KG-1, and HH cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD96 recombinant monoclonal antibody [BLR065G] (Product # A700-065 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD96 by immunocytochemistry.Sample: FFPE section of MOLT-4 cells.Antibody: Rabbit anti-CD96 recombinant monoclonal antibody [BLR065G] (Product # A700-065; lot 1) used at 1:200.Secondary: HRP-conjugated goat anti-rabbit IgG (Product # A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD96 by immunohistochemistry. AntibodySample: FFPE section of ovarian carcinoma.Antibody: Rabbit anti-CD96 recombinant monoclonal antibody (BLR065G) (A700-065 lot 1) used at 1:200.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD96 by immunohistochemistry.Sample: FFPE section of tonsil.Antibody: Rabbit anti-CD96 recombinant monoclonal antibody [BLR065G] (Product # A700-065 lot 1) used at 1:200.Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human CD96 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MOLT-4 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD96 recombinant monoclonal antibody [BLR065G] (Product # A700-065 lot 1) used for IP at 6 µL per reaction. CD96 was also immunoprecipitated by rabbit anti-CD96 recombinant monoclonal antibody [BL-855-3C7]. For blotting immunoprecipitated CD96 (Product # A700-065) was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.