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LearnPA5-29871
antibody from Invitrogen Antibodies
Targeting: RUVBL2
ECP51, INO80J, Reptin52, RVB2, TIH2, TIP48, TIP49b
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-29871 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RUVBL2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (91%), Xenopus laevis (96%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.89 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references PRMT5-Dependent Methylation of the TIP60 Coactivator RUVBL1 Is a Key Regulator of Homologous Recombination.
Clarke TL, Sanchez-Bailon MP, Chiang K, Reynolds JJ, Herrero-Ruiz J, Bandeiras TM, Matias PM, Maslen SL, Skehel JM, Stewart GS, Davies CC
Molecular cell 2017 Mar 2;65(5):900-916.e7
Molecular cell 2017 Mar 2;65(5):900-916.e7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RUVBL2 Polyclonal Antibody (Product # PA5-29871). Sample (30 µg of whole cell lysate). Lane A: H1299. 10% SDS PAGE. RUVBL2 Polyclonal Antibody (Product # PA5-29871) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RUVBL2 in paraformaldehyde-fixed A549 cells using a RUVBL2 polyclonal antibody (Product # PA5-29871) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RUVBL2 Polyclonal Antibody detects RUVBL2 protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. RUVBL2 Polyclonal Antibody (Product # PA5-29871) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of RUVBL2 was performed in 293T whole cell extracts using 5 µg of RUVBL2 Polyclonal Antibody (Product # PA5-29871). Samples were transferred to a membrane and probed with RUVBL2 Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
