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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [2]
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- Product number
- 12-9742-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EVA1 Monoclonal Antibody (G9P3-1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody G9P3-1 reacts with human and mouse epithelial V-like antigen 1 (EVA1), also known as myelin protein zero-like 2 (MPZL2). EVA1 is a low affinity adhesion type I transmembrane glycoprotein that bears an immunolobulin V-type domain. This glycoprotein is expressed on some epithelial tissues and also on immature thymocytes in the DN3 stage. The DN3 thymocytes are characterized by CD4 and CD8 double negative cells that are CD25 positive and CD44 negative. EVA1 has a role in immature thymocyte development, and thymic regeneration.
- Conjugate
- Yellow dye
- Antibody clone number
- G9P3-1
- Concentration
- 0.2 mg/mL
Submitted references A monoclonal antibody against the extracellular domain of mouse and human epithelial V-like antigen 1 reveals a restricted expression pattern among CD4- CD8- thymocytes.
Lymphoid EVA1 expression is required for DN1-DN3 thymocytes transition.
Molecular dissection of prethymic progenitor entry into the T lymphocyte developmental pathway.
Garabatos N, Blanco J, Fandos C, Lopez E, Santamaria P, Ruiz A, Perez-Vidakovics ML, Benveniste P, Galkin O, Zuñiga-Pflucker JC, Serra P
Monoclonal antibodies in immunodiagnosis and immunotherapy 2014 Oct;33(5):305-11
Monoclonal antibodies in immunodiagnosis and immunotherapy 2014 Oct;33(5):305-11
Lymphoid EVA1 expression is required for DN1-DN3 thymocytes transition.
Iacovelli S, Iosue I, Di Cesare S, Guttinger M
PloS one 2009 Oct 23;4(10):e7586
PloS one 2009 Oct 23;4(10):e7586
Molecular dissection of prethymic progenitor entry into the T lymphocyte developmental pathway.
Tydell CC, David-Fung ES, Moore JE, Rowen L, Taghon T, Rothenberg EV
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 1;179(1):421-38
Journal of immunology (Baltimore, Md. : 1950) 2007 Jul 1;179(1):421-38
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C57BL/6 thymocytes were stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82), Anti-Mouse CD8 FITC (Product # 11-0081-82), Anti-Human/Mouse CD44 PE-eFluor® 610 (Product # 61-0441-82), Anti-Mouse CD25 PerCP-Cyanine5-5 (Product # 45-0251-82), and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human/Mouse EVA1 PE. Isotype control (blue histogram) or EVA1 (purple histogram) staining on DN3 cells (CD4-CD8-CD44-CD25+) are shown and compared to EVA1 staining on DN1 cells (CD4-CD8-CD44+CD25-) (orange histogram). Viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- C57BL/6 thymocytes were stained with Anti-Mouse CD4 eFluor® 450 (Product # 48-0042-82), Anti-Mouse CD8 FITC (Product # 11-0081-82), Anti-Human/Mouse CD44 PE-eFluor® 610 (Product # 61-0441-82), Anti-Mouse CD25 PerCP-Cyanine5-5 (Product # 45-0251-82), and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) or Anti-Human/Mouse EVA1 PE. Isotype control (blue histogram) or EVA1 (purple histogram) staining on DN3 cells (CD4-CD8-CD44-CD25+) are shown and compared to EVA1 staining on DN1 cells (CD4-CD8-CD44+CD25-) (orange histogram). Viable cells, as determined by Fixable Viability Dye eFluor® 506 (Product # 65-0866-14), were used for analysis.
- Conjugate
- Yellow dye
