PAB29497

antibody from Abnova Corporation

Targeting: FMR1 FMRP, FRAXA, MGC87458, POF, POF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29497

Provider

Abnova Corporation

Product name

FMR1 polyclonal antibody

Antibody type

Polyclonal

Description

FMR1 polyclonal antibody raised against recombinant human FMR1.

Antigen sequence

FKGNDDHSRTDNRPRNPREAKGRTTDGSLQIRVDC
NNERSVHTKTLQNTSSEGSRLRTGKDRNQKKEKPD
SVDGQQPLVNGVP


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot (Cell lysate) analysis of Lane 1: RT-4 cell lysate, Lane 2: U-251 MG cell lysate with FMR1 polyclonal antibody (Cat# PAB29497) at 1:250 - 1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of U-251 MG cells with FMR1 polyclonal antibody (Cat# PAB29497) under 1-4 ug/mL working concentration shows positivity in cytoplasm. Antibody staining is shown in green.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with FMR1 polyclonal antibody (Cat# PAB29497) shows strong cytoplasmic positivity in neuronal cells at 1:200 - 1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)