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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- MA5-15499 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FMRP Monoclonal Antibody (4G9)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15499 targets FMR1 in IF, IHC, and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 4G9
- Concentration
- Conc. Not Determined
Submitted references The homophilic receptor PTPRK selectively dephosphorylates multiple junctional regulators to promote cell-cell adhesion.
New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules.
Fearnley GW, Young KA, Edgar JR, Antrobus R, Hay IM, Liang WC, Martinez-Martin N, Lin W, Deane JE, Sharpe HJ
eLife 2019 Mar 29;8
eLife 2019 Mar 29;8
New roles for the de-ubiquitylating enzyme OTUD4 in an RNA-protein network and RNA granules.
Das R, Schwintzer L, Vinopal S, Aguado Roca E, Sylvester M, Oprisoreanu AM, Schoch S, Bradke F, Broemer M
Journal of cell science 2019 Jun 17;132(12)
Journal of cell science 2019 Jun 17;132(12)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HEK-293 (Lane 1), SH-SY5Y (Lane 2), Jurkat (Lane 3), K-562 (Lane 4), U-87 MG (Lane 5) and HeLa (Lane 6). The blot was probed with Anti-FMRP Monoclonal Antibody (Product # MA5-15499, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 71 kDa band corresponding to FMRP was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of FMRP was achieved by transfecting SH-SY5Y with FMRP specific siRNAs (Silencer® select Product # s5317, s5316 ). Western blot analysis (Fig. a) was performed using whole cell extracts from the FMRP knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with FMRP Monoclonal Antibody (Product # MA5-15499, 1:2000 dilution) and Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to FMRP.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using FMR1 monoclonal antibody (Product # MA5-15499) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of FMRP was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with FMRP Mouse Monoclonal Antibody (Product # MA5-15499) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain tissues using FMR1 monoclonal antibody (Product # MA5-15499) followed with DAB staining
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
