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LearnOAAB02286
antibody from Aviva Systems Biology
Targeting: PRPF19
hPSO4, NMP200, PRP19, PSO4, SNEV, UBOX4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- OAAB02286 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- PRPF19 antibody - N-terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of PRPF19 Antibody (N-term) in MCF-7 cell lysates (35ug/lane). PRPF19 (arrow) was detected using the purified Pab.
- Sample type
- MCF-7 cell line lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of PRPF19 Antibody (N-term) in mouse brain tissue lysates (35ug/lane). PRPF19 (arrow) was detected using the purified Pab.
- Sample type
- mouse brain tissue lysates
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- PRPF19 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the PRPF19 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
- Sample type
- lung carcinoma
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- PRPF19 Antibody (N-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Sample type
- MCF-7 cells
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
