ABIN872476

antibody from antibodies-online

Targeting: CLDND2 MGC33839

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ABIN872476

Provider

antibodies-online

Product name

anti-Claudin Domain Containing 2 (CLDND2) antibody

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide derived from human CLDND2

Description

purified by Protein A and peptide affinity chromatography

Reactivity

Human, Mouse, Rat, Bovine, Porcine

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

1 μg/μL

Storage

Store at -20°C for 12 months

Western blot

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Mouse brain lysates probed with Anti-CLDND2 Polyclonal Antibody, Unconjugated (ABIN872476) at 1:300 in 4?C. Followed by conjugation to secondary antibody at 1:5000 90min in 37?C.

Immunohistochemistry

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval with 0.4% pepsin at 37°C for 30min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-CLDND2 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining

Supportive validation

Submitted by

antibodies-online (provider)

Main image

Experimental details

paraformaldehyde-fixed, paraffin embedded human kidney tissue; Antigen retrieval with 0.4% pepsin at 37°C for 30min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-CLDND2 Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining