GTX83993

antibody from GeneTex

Targeting: NT5DC1 dJ486I3.1, MGC24302, NT5C2L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX83993

Provider

GeneTex

Proper citation

GeneTex Cat#GTX83993, RRID:AB_10726843

Product name

NT5DC1 antibody [2B12]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Storage

For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NT5DC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NT5DC1.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Anti-NT5DC1 mouse monoclonal antibody (GTX83993) immunofluorescent staining of COS7 cells transiently transfected with NT5DC1

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83993)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83993)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83993)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83993)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83993)

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NT5DC1 mouse monoclonal antibody. (GTX83993)

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-NT5DC1 antibody(GTX83993),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

HEK293T cells transfected with either RC211087 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NT5DC1 antibody(GTX83993), and then analyzed by flow cytometry.