ADI-905-792-100

antibody from Enzo Life Sciences

Targeting: GHSR

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ADI-905-792-100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ADI-905-792-100, RRID:AB_2039131

Product name

Ghrelin receptor GHS-R, pAb

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide affinity purified. Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis: MW marker (1) and 20µg rat heart extract (2) probed with Ghrelin Receptor (GHS-R) pAb 8.5µg/ml.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Immunohistochemistry analysis: Human anterior pituitary, formalin-fixed, paraffin-embedded, and stained with Ghrelin Receptor (GHS-R) pAb at 40µg/ml. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Membrane ELISA: Lewis rat heart membranes (5µg/well) were treated with 1 µM concentrations of agonist (Ghrelin) and probed with Ghrelin Receptor (GHS-R) pAb (1:250 to 1:2000 of a 1µg/µL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=4).