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Learn10769-1-AP
antibody from Proteintech Group
Targeting: SEPTIN9
AF17q25, KIAA0991, MSF, MSF1, PNUTL4, SEPT9, SeptD1
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- 10769-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#10769-1-AP, RRID:AB_2185012
- Product name
- Septin 9 antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Septin 9 antibody (Cat. #10769-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Septin-microtubule association via a motif unique to isoform 1 of septin 9 tunes stress fibers.
Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.
Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.
A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Septin 9 isoforms promote tumorigenesis in mammary epithelial cells by increasing migration and ECM degradation through metalloproteinase secretion at focal adhesions.
A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.
Characterization of septin expression in normal and fibrotic kidneys.
Septin filament coalignment with microtubules depends on SEPT9_i1 and tubulin polyglutamylation, and is an early feature of acquired cell resistance to paclitaxel.
Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin.
Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.
Septins suppress the release of vaccinia virus from infected cells.
Septins are critical regulators of osteoclastic bone resorption.
Fluorescence microscopy of actin- and microtubule-associated septins in mammalian cells.
Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport.
Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.
Deciphering the rules governing assembly order of mammalian septin complexes.
Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci.
Kuzmić M, Castro Linares G, Leischner Fialová J, Iv F, Salaün D, Llewellyn A, Gomes M, Belhabib M, Liu Y, Asano K, Rodrigues M, Isnardon D, Tachibana T, Koenderink GH, Badache A, Mavrakis M, Verdier-Pinard P
Journal of cell science 2022 Jan 1;135(1)
Journal of cell science 2022 Jan 1;135(1)
Cdc42 and its BORG2 and BORG3 effectors control the subcellular localization of septins between actin stress fibers and microtubules.
Salameh J, Cantaloube I, Benoit B, Poüs C, Baillet A
Current biology : CB 2021 Sep 27;31(18):4088-4103.e5
Current biology : CB 2021 Sep 27;31(18):4088-4103.e5
Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties.
Soroor F, Kim MS, Palander O, Balachandran Y, Collins RF, Benlekbir S, Rubinstein JL, Trimble WS
Molecular biology of the cell 2021 Feb 1;32(3):289-300
Molecular biology of the cell 2021 Feb 1;32(3):289-300
A septin GTPase scaffold of dynein-dynactin motors triggers retrograde lysosome transport.
Kesisova IA, Robinson BP, Spiliotis ET
The Journal of cell biology 2021 Feb 1;220(2)
The Journal of cell biology 2021 Feb 1;220(2)
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms.
Iv F, Martins CS, Castro-Linares G, Taveneau C, Barbier P, Verdier-Pinard P, Camoin L, Audebert S, Tsai FC, Ramond L, Llewellyn A, Belhabib M, Nakazawa K, Di Cicco A, Vincentelli R, Wenger J, Cabantous S, Koenderink GH, Bertin A, Mavrakis M
Journal of cell science 2021 Aug 1;134(15)
Journal of cell science 2021 Aug 1;134(15)
Septin 9 isoforms promote tumorigenesis in mammary epithelial cells by increasing migration and ECM degradation through metalloproteinase secretion at focal adhesions.
Marcus J, Bejerano-Sagie M, Patterson N, Bagchi S, Verkhusha VV, Connolly D, Goldberg GL, Golden A, Sharma VP, Condeelis J, Montagna C
Oncogene 2019 Jul;38(30):5839-5859
Oncogene 2019 Jul;38(30):5839-5859
A Septin Double Ring Controls the Spatiotemporal Organization of the ESCRT Machinery in Cytokinetic Abscission.
Karasmanis EP, Hwang D, Nakos K, Bowen JR, Angelis D, Spiliotis ET
Current biology : CB 2019 Jul 8;29(13):2174-2182.e7
Current biology : CB 2019 Jul 8;29(13):2174-2182.e7
Characterization of septin expression in normal and fibrotic kidneys.
Neubauer K, Neubauer B, Seidl M, Zieger B
Cytoskeleton (Hoboken, N.J.) 2019 Jan;76(1):143-153
Cytoskeleton (Hoboken, N.J.) 2019 Jan;76(1):143-153
Septin filament coalignment with microtubules depends on SEPT9_i1 and tubulin polyglutamylation, and is an early feature of acquired cell resistance to paclitaxel.
Targa B, Klipfel L, Cantaloube I, Salameh J, Benoit B, Poüs C, Baillet A
Cell death & disease 2019 Jan 22;10(2):54
Cell death & disease 2019 Jan 22;10(2):54
Sorting nexin-21 is a scaffold for the endosomal recruitment of huntingtin.
Danson CM, Pearson N, Heesom KJ, Cullen PJ
Journal of cell science 2018 Sep 10;131(17)
Journal of cell science 2018 Sep 10;131(17)
Polarity of Neuronal Membrane Traffic Requires Sorting of Kinesin Motor Cargo during Entry into Dendrites by a Microtubule-Associated Septin.
Karasmanis EP, Phan CT, Angelis D, Kesisova IA, Hoogenraad CC, McKenney RJ, Spiliotis ET
Developmental cell 2018 Jul 16;46(2):204-218.e7
Developmental cell 2018 Jul 16;46(2):204-218.e7
Septins suppress the release of vaccinia virus from infected cells.
Pfanzelter J, Mostowy S, Way M
The Journal of cell biology 2018 Aug 6;217(8):2911-2929
The Journal of cell biology 2018 Aug 6;217(8):2911-2929
Septins are critical regulators of osteoclastic bone resorption.
Møller AMJ, Füchtbauer EM, Brüel A, Andersen TL, Borggaard XG, Pavlos NJ, Thomsen JS, Pedersen FS, Delaisse JM, Søe K
Scientific reports 2018 Aug 29;8(1):13016
Scientific reports 2018 Aug 29;8(1):13016
Fluorescence microscopy of actin- and microtubule-associated septins in mammalian cells.
Spiliotis ET, Karasmanis EP, Dolat L
Methods in cell biology 2016;136:243-68
Methods in cell biology 2016;136:243-68
Septin 9 interacts with kinesin KIF17 and interferes with the mechanism of NMDA receptor cargo binding and transport.
Bai X, Karasmanis EP, Spiliotis ET
Molecular biology of the cell 2016 Mar 15;27(6):897-906
Molecular biology of the cell 2016 Mar 15;27(6):897-906
Genetic deletion of SEPT7 reveals a cell type-specific role of septins in microtubule destabilization for the completion of cytokinesis.
Menon MB, Sawada A, Chaturvedi A, Mishra P, Schuster-Gossler K, Galla M, Schambach A, Gossler A, Förster R, Heuser M, Kotlyarov A, Kinoshita M, Gaestel M
PLoS genetics 2014 Aug;10(8):e1004558
PLoS genetics 2014 Aug;10(8):e1004558
Deciphering the rules governing assembly order of mammalian septin complexes.
Sellin ME, Sandblad L, Stenmark S, Gullberg M
Molecular biology of the cell 2011 Sep;22(17):3152-64
Molecular biology of the cell 2011 Sep;22(17):3152-64
Quantitative and integrative proteome analysis of peripheral nerve myelin identifies novel myelin proteins and candidate neuropathy loci.
Patzig J, Jahn O, Tenzer S, Wichert SP, de Monasterio-Schrader P, Rosfa S, Kuharev J, Yan K, Bormuth I, Bremer J, Aguzzi A, Orfaniotou F, Hesse D, Schwab MH, Möbius W, Nave KA, Werner HB
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Nov 9;31(45):16369-86
The Journal of neuroscience : the official journal of the Society for Neuroscience 2011 Nov 9;31(45):16369-86
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10769-1-AP(SEPT9 antibody) at dilution of 1:600
- Sample type
- tissue
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using SEPT9 antibody 10769-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 10769-1-AP(SEPT9 antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human pancreas cancer using 10769-1-AP(SEPT9 antibody) at dilution of 1:50 (under 40x lens)
- Sample type
- tissue
