OAAB01685

antibody from Aviva Systems Biology

Targeting: P4HB DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PO4DB, PO4HB, PROHB

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

OAAB01685

Provider

Aviva Systems Biology

Product name

P4HB antibody - C-terminal region

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Vial size

400ul

Storage

Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Western blot analysis of P4HB Antibody (C-term) in CEM,HepG2,A2058 cell lysates (35ug/lane). P4HB (arrow) was detected using the purified Pab.(2ug/ml,)

Sample type

CEM,HepG2,A2058 cell line lysates

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with P4HB Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Sample type

human hepatocarcinoma

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol

Flow cytometry

Supportive validation

Submitted by

Aviva Systems Biology (provider)

Main image

Experimental details

Flow cytometric analysis of K562 cells using P4HB Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Sample type

K562 cells

Primary Ab dilution

1.0 µg/mL

Protocol

Protocol