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LearnH00005034-D01
antibody from Abnova Corporation
Targeting: P4HB
DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PO4DB, PO4HB, PROHB
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00005034-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005034-D01, RRID:AB_10717367
- Product name
- P4HB MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human P4HB protein.
- Antigen sequence
MLRRALLCLAVAALVRADAPEEEDHVLVLRKSNFA
EALAAHKYLLVEFYAPWCGHCKALAPEYAKAAGKL
KAEGSEIRLAKVDATEESDLAQQYGVRGYPTIKFF
RNGDTASPKEYTAGREADDIVNWLKKRTGPAATTL
PDGAAAESLVESSEVAVIGFFKDVESDSAKQFLQA
AEAIDDIPFGITSNSDVFSKYQLDKDGVVLFKKFD
EGRNNFEGEVTKENLLDFIKHNQLPLVIEFTEQTA
PKIFGGEIKTHILLFLPKSVSDYDGKLSNFKTAAE
SFKGKILFIFIDSDHTDNQRILEFFGLKKEECPAV
RLITLEEEMTKYKPESEELTAERITEFCHRFLEGK
IKPHLMSQELPEDWDKQPVKVLVGKNFEDVAFDEK
KNVFVEFYAPWCGHCKQLAPIWDKLGETYKDHENI
VIAKMDSTANEVEAVKVHSFPTLKFFPASADRTVI
DYNGERTLDGFKKFLESGGQDGAGDDDDLEDLEEA
EEPDMEEDDDQKAVKDEL- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of P4HB expression in transfected 293T cell line (H00005034-T02) by P4HB MaxPab polyclonal antibody.Lane 1: P4HB transfected lysate(57.10 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- P4HB MaxPab rabbit polyclonal antibody. Western Blot analysis of P4HB expression in mouse kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to P4HB on HeLa cell. [antibody concentration 5 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of P4HB transfected lysate using anti-P4HB MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with P4HB purified MaxPab mouse polyclonal antibody (B01P) (H00005034-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
