Explore
Validate
LearnLS-B15337
antibody from LSBio
Targeting: P4HB
DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PO4DB, PO4HB, PROHB
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunoprecipitation [4]
Submit
Validation data
Reference
Comment
Report error
- Product number
- LS-B15337 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ PDI / P4HB Antibody LS-B15337
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at -20°C. Avoid freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using P4HB antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and P4HB knockout (KO) HeLa cells, using P4HB antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from normal (control) and P4HB knockout (KO) HeLa cells, using P4HB antibodyat 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 1s.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody. Western blot was performed from the immunoprecipitate using P4HB antibodyat a dilition of 1:500.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody. Western blot was performed from the immunoprecipitate using P4HB antibodyat a dilition of 1:500.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody. Western blot was performed from the immunoprecipitate using P4HB antibodyat a dilition of 1:500.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody. Western blot was performed from the immunoprecipitate using P4HB antibodyat a dilition of 1:500.
