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LearnLS-C818204
antibody from LSBio
Targeting: P4HB
DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PO4DB, PO4HB, PROHB
Western blot
ELISA
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C818204 - Provider product page
- Provider
- LSBio
- Product name
- PDI / P4HB Antibody (Preservative Free) LS-C818204
- Antibody type
- Polyclonal
- Description
- Protein A affinity chromatography
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-P4HB rabbit polyclonal antibody at 1:1000 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: HeLa Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: A431 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- P4HB was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-P4HB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-P4HB rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- P4HB was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. 4 uL anti-P4HB rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-P4HB rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 57 kDa. Observed band size: 57 kDa.
