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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX117656 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX117656, RRID:AB_10619628
- Product name
- EBF3 antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE GTX117656 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- EBF3 antibody [C1C3] detects EBF3 protein at nucleus by immunofluorescent analysis.Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EBF3 protein stained by EBF3 antibody [C1C3] (GTX117656) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of EBF3 protein from U87-MG whole cell extracts using 5 £gg of EBF3 antibody [C1C3] (GTX117656).Western blot analysis was performed using EBF3 antibody [C1C3] (GTX117656).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
