Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 21148-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EBF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: CNQNCLKNA GNPRDMRRFQ VVVSTTVNVD GHVLAVSDNM FVHNNSKHGR RARRLDPSEA TPCIKAISPS EGWTTGGATV IIIGDNFFDG LQVVFGTMLV WSELITPHAI RVQTPPRHIP GVVEVTLSYK SKQFCKGAPG RFVYTALNEP TIDYGFQRLQ KVIPRHPGDP ERLPKEVLLK RAADLVEALY GMPHNNQEII LKRAADIAEA LYSVPRNHNQ IPTLGNNPAH TGMMGVNSFS SQLAVNVSET SQANDQVGYS RNTSSVSPRG YVPSSTPQQS NYNTVSTSMN GYGSGAMASL GVPGSPGFLN GSSANSPYGM KQKSAFAPVV RPQASPPPSC TSANGNGLQA M (194-543 aa encoded by BC126130)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with (Product # 21148-1-AP) (EBF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 21148-1-AP (EBF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- DU 145 cells were subjected to SDS PAGE followed by western blot with 21148-1-AP (EBF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 21148-1-AP (EBF3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
