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LearnPAB22777
antibody from Abnova Corporation
Targeting: RSPH4A
CILD11, dJ412I7.1, FLJ37974, RSHL3, RSPH6B
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB22777 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB22777, RRID:AB_10962658
- Product name
- RSHL3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against recombinant RSHL3.
- Antigen sequence
DVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGG
SEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLS
NMLTKI- Isotype
- IgG
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RSHL3 polyclonal antibody (Cat # PAB22777) at 1:250-1:500 dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human cell line A-431 with RSHL3 polyclonal antibody (Cat # PAB22777) at 1-4 ug/mL dilution shows positivity in nucleus but not nucleoli.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human kidney with RSHL3 polyclonal antibody (Cat # PAB22777) shows strong cytoplasmic positivity in cells in tubules at 1:50-1:200 dilution.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
