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- Western blot [3]
- ELISA [1]
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- Product number
- H00003841-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003841-M01, RRID:AB_540076
- Product name
- Mouse Monoclonal KPNA5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. KPNA5 - karyopherin alpha 5 (importin alpha 6)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references BIG3 Inhibits the Estrogen-Dependent Nuclear Translocation of PHB2 via Multiple Karyopherin-Alpha Proteins in Breast Cancer Cells.
Kim NH, Yoshimaru T, Chen YA, Matsuo T, Komatsu M, Miyoshi Y, Tanaka E, Sasa M, Mizuguchi K, Katagiri T
PloS one 2015;10(6):e0127707
PloS one 2015;10(6):e0127707
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - Analysis of KPNA5 over-expressed 293 cell line, cotransfected with KPNA5 Validated Chimera RNAi ( Cat # H00003841-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with KPNA5 monoclonal antibody (M01), clone 1D2 (Cat # H00003841-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - Analysis of KPNA5 expression in transfected 293T cell line by KPNA5 monoclonal antibody (M01), clone 1D2.Lane 1: KPNA5 transfected lysate(60.7 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KPNA5 Antibody (1D2) [H00003841-M01] - KPNA5 monoclonal antibody (M01), clone 1D2 Analysis of KPNA5 expression in HepG2.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: KPNA5 Antibody (1D2) [H00003841-M01] - Detection limit for recombinant GST tagged KPNA5 is approximately 3ng/ml as a capture antibody.
