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Western blot
Immunohistochemistry
Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- HPA037696 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA037696, RRID:AB_2675616
- Product name
- Anti-RFX6
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human RFX6, Gene description: regulatory factor X, 6, Alternative Gene Names: dJ955L16.1, MGC33442, RFXDC1, Validated applications: WB, IHC, ChIP, Uniprot ID: Q8HWS3, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Loss of RREB1 in pancreatic beta cells reduces cellular insulin content and affects endocrine cell gene expression
Mattis K, Krentz N, Metzendorf C, Abaitua F, Spigelman A, Sun H, Ikle J, Thaman S, Rottner A, Bautista A, Mazzaferro E, Perez-Alcantara M, Manning Fox J, Torres J, Wesolowska-Andersen A, Yu G, Mahajan A, Larsson A, MacDonald P, Davies B, den Hoed M, Gloyn A
Diabetologia 2023;66(4):674-694
Diabetologia 2023;66(4):674-694
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and RFX6 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406471).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Main image
- Experimental details
- ChIP-Exo-Seq composite graph for Anti-RFX6 (HPA037696, Lot 000031588) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
