ACC-002-200UL

antibody from Invitrogen Antibodies

Targeting: CACNA1B CACNL1A5, CACNN, Cav2.2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

ACC-002-200UL

Provider

Invitrogen Antibodies

Product name

CACNA1B (CaV2.2) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

200 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membranes: - 1. Anti-CACNA1B (CaV2.2) Antibody (#ACC-002), (1:200). 2. Anti-CACNA1B (CaV2.2) Antibody , preincubated with the control antigen.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of CaV2.2 in rat DRG primary culture - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized rat dorsal root ganglion (DRG) primary culture. A. Cells were stained using Anti-CACNA1B (CaV2.2) Antibody (#ACC-002), (1:200) followed by goat Anti-rabbit-AlexaFluor-555 secondary Antibody . B. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of CaV2.2 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum with Anti-CACNA1B (CaV2.2) Antibody (#ACC-002), (1:100).A. CaV2.2 (red) appears in Purkinje cells (arrows) and is distributed diffusely in the molecular layer (Mol). B. Staining of Purkinje cells with mouse Anti-Calbindin 28K (green) demonstrates the restriction of CaV2.2 to cell bodies but not to dendrites in the molecular layer. C. Merged image of panels A and B.