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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- TA805804 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA805804, RRID:AB_2627868
- Product name
- ROS1 mouse monoclonal antibody, clone OTI1A1 (formerly 1A1)
- Antibody type
- Monoclonal
- Description
- ROS1 mouse monoclonal antibody, clone OTI1A1 (formerly 1A1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ROS1
- Isotype
- IgG
- Antibody clone number
- OTI1A1
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (RC220652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 2 different cell lines by using anti-ROS1 monoclonal antibody.(1:500)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (TA805804)(Left). The right is HELA cells as negative control.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograft using TA805804 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min).
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded non-small cell lung cancer tissues (case1 was ROS1-positive, Case2 was ROS1-negative) using TA805804 (0.2ug/ml). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min)
- Validation comment
- IHC
