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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- CF805804 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ROS1 Monoclonal Antibody (OTI1A1), TrueMAB™
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI1A1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (RC220652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ROS1 (RC220652, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-ROS1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35 µg) from 2 different cell lines by using anti-ROS1 monoclonal antibody. (1:500)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HCC78 cells using anti-ROS1 mouse monoclonal antibody (TA805804)(Left). The right is HELA cells as negative control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded HCC78 (left) and HeLa (right) xenograftusing TA805804 (0.2 µg/mL). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded non-small cell lung cancer tissues (case1 was ROS1-positive, Case2 was ROS1-negative) using TA805804 (0.2 µg/mL). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3 min
