PA5-97143

antibody from Invitrogen Antibodies

Targeting: PLK3 CNK, FNK, PRK

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-97143

Provider

Invitrogen Antibodies

Product name

PLK3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: VLFNDGTHMA LSANRKTVHY NPTSTKHFSF SVGAVPRALQ PQLGILRYFA SYMEQHLMKG GDLPSVEEVE VPAPPLLLQW VKTDQALLML FSDGTVQVNF YGDHTKLILS GWEPLLVTFV ARNRSACTYL ASHLRQLGCS PDLRQRLRYA LRLLRDRSPA; Positive Samples: A-549, HT-1080, SKOV3, 293T, U2OS, Mouse liver, Rat liver; Cellular Location: Cytoplasm, Golgi apparatus, Nucleus, centrosome, cytoskeleton, microtubule organizing center, nucleolus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Natural COA water inhibits mitochondrial ROS-mediated apoptosis through Plk3 downregulation under STZ diabetic stress in pancreatic β-cell lines.

Lee J, Chung JO, Park SY, Rajamohan N, Singh A, Kim J, Lowe VJ, Lee S
Biochemistry and biophysics reports 2022 Jul;30:101247

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PLK3 in extracts of various cell lines using PLK3 Polyclonal Antibody (Product # PA5-97143) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

The mitochondria-mediated apoptotic cell death via streptozotocin (STZ) in beta cells is inhibited by natural COA water. (A) Beta TC-6 islet cells were cultured in a medium prepared with general water (Control) or natural COA water and then treated with streptozotocin for 72 h. Cell lysates were analyzed by immunoblotting with the antibodies indicated. (B) Beta TC-6 cells were cultured in a medium prepared with general water (Control) or natural COA water and then treated by H 2 O 2 (10 muM) for 24 h. Cell lysates were analyzed by immunoblotting with the antibodies indicated. (C) Pancreatic Beta TC-6 islet cells were cultured with indicated waters and then treated by Streptozotocin or NAC (1 mM). Cell lysates were analyzed by immunoblotting with the antibodies indicated. (D) Beta TC-6 cells were cultured in a medium prepared with general water or natural COA water and then treated with streptozotocin for 72 h. And some of these cells were treated with MG132 (10 muM, for 4 h) in a medium prepared with natural COA water. Cell lysates were analyzed by immunoblotting with the antibodies indicated. (E, F) Beta TC-6 cells were cultured in a medium prepared with general water (Control) or natural COA water and then treated with streptozotocin for 18 h. The cells were stained with MitoSOX (5 muM) for 30 min and then observed by fluorescence microscopy. Representative images of mitochondrial ROS in the Beta TC-6 cells (E). Quantification of cleaved Caspase-9, cleaved Caspase-3, cleave