Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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- Product number
- GTX103247 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103247, RRID:AB_1949643
- Product name
- Amphiphysin antibody [N1N2], N-term
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
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- Experimental details
- Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis.A. 50 ?g mouse brain lysate/extract 7.5 % SDS-PAGEAmphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:1000
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- Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis.A. 50 ?g rat brain lysate/extract 5 % SDS-PAGEAmphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:500
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- Experimental details
- Mouse tissue extract (50 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin antibody [N1N2], N-term (GTX103247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Various tissue extracts (50 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Amphiphysin antibody [N1N2], N-term (GTX103247) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- Amphiphysin antibody [N1N2], N-term detects Amphiphysin protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Amphiphysin stained by Amphiphysin antibody [N1N2], N-term (GTX103247) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
Supportive validation
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- Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:500.
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- Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:500.
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- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Amphiphysin(GTX103247) antibody at 1:100 dilution.
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- Experimental details
- Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin antibody [N1N2], N-term (GTX103247) dilution: 1:500.