PA5-21483

antibody from Invitrogen Antibodies

Targeting: AMPH

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-21483

Provider

Invitrogen Antibodies

Product name

Amphiphysin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: mouse hippocampus, mouse brain, rat brain.

Concentration

0.9 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Amphiphysiniphysin isoform 1 using 50 µg mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an Amphiphysiniphysin isoform 1 polyclonal antibody (Product # PA5-21483) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Amphiphysiniphysin isoform 1 using 50 µg rat brain lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with an Amphiphysiniphysin isoform 1 polyclonal antibody (Product # PA5-21483) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Amphiphysin was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Amphiphysin Polyclonal Antibody (Product # PA5-21483) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Amphiphysin was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a Amphiphysin Polyclonal Antibody (Product # PA5-21483) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Amphiphysin was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Amphiphysin Polyclonal Antibody (Product # PA5-21483) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Amphiphysin Polyclonal Antibody detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Amphiphysin Polyclonal Antibody (Product # PA5-21483) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Amphiphysin (Product # PA5-21483) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Amphiphysin Polyclonal Antibody detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded RT2 xenograft. Amphiphysin Polyclonal Antibody (Product # PA5-21483) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Amphiphysin Polyclonal Antibody detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Amphiphysin Polyclonal Antibody (Product # PA5-21483) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.