- GTX110815 - GeneTex ATP6V1A antibody

Submitted references TMEM55B contributes to lysosomal homeostasis and amino acid-induced mTORC1 activation.

Hashimoto Y, Shirane M, Nakayama KI
Genes to cells : devoted to molecular & cellular mechanisms 2018 Jun;23(6):418-434

Activity-independent targeting of mTOR to lysosomes in primary osteoclasts.

Wang A, Carraro-Lacroix LR, Owen C, Gao B, Corey PN, Tyrrell P, Brumell JH, Voronov I
Scientific reports 2017 Jun 7;7(1):3005

mTORC1 and muscle regeneration are regulated by the LINC00961-encoded SPAR polypeptide.

Matsumoto A, Pasut A, Matsumoto M, Yamashita R, Fung J, Monteleone E, Saghatelian A, Nakayama KI, Clohessy JG, Pandolfi PP
Nature 2017 Jan 12;541(7636):228-232

Neuronal ceroid lipofuscinosis with DNAJC5/CSPα mutation has PPT1 pathology and exhibit aberrant protein palmitoylation.

Henderson MX, Wirak GS, Zhang YQ, Dai F, Ginsberg SD, Dolzhanskaya N, Staropoli JF, Nijssen PC, Lam TT, Roth AF, Davis NG, Dawson G, Velinov M, Chandra SS
Acta neuropathologica 2016 Apr;131(4):621-37

SLC38A9 is a component of the lysosomal amino acid sensing machinery that controls mTORC1.

Rebsamen M, Pochini L, Stasyk T, de Araújo ME, Galluccio M, Kandasamy RK, Snijder B, Fauster A, Rudashevskaya EL, Bruckner M, Scorzoni S, Filipek PA, Huber KV, Bigenzahn JW, Heinz LX, Kraft C, Bennett KL, Indiveri C, Huber LA, Superti-Furga G
Nature 2015 Mar 26;519(7544):477-81

Metabolism. Differential regulation of mTORC1 by leucine and glutamine.

Jewell JL, Kim YC, Russell RC, Yu FX, Park HW, Plouffe SW, Tagliabracci VS, Guan KL
Science (New York, N.Y.) 2015 Jan 9;347(6218):194-8

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: >50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 41822

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ?g of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX110815 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 (GTX27900) 7.5% SDS PAGE GTX110815 diluted at 1:10000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATP6V1A antibody detects ATP6V1A protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted by 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody (GTX110815) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of methanol-fixed A431, using ATP6V1A(GTX110815) antibody at 1:500 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of ATP6V1A protein from HeLa whole cell extracts using 5 £gg of ATP6V1A antibody (GTX110815).Western blot analysis was performed using ATP6V1A antibody (GTX110815).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: ATP6V1A antibody detects ATP6V1A protein at cytoplasm on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ATP6V1A antibody (GTX110815) diluted at 1:500.

GTX110815

Antibody data