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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00000523-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000523-M02, RRID:AB_2062369
- Product name
- Mouse Monoclonal ATP6V1A Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. Reacts with ATPase, H+ transporting, lysosomal 70kDa, V1 subunit A.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Extracellular and Luminal pH Regulation by Vacuolar H+-ATPase Isoform Expression and Targeting to the Plasma Membrane and Endosomes.
Smith GA, Howell GJ, Phillips C, Muench SP, Ponnambalam S, Harrison MA
The Journal of biological chemistry 2016 Apr 15;291(16):8500-15
The Journal of biological chemistry 2016 Apr 15;291(16):8500-15
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of ATP6V1A expression in transfected 293T cell line by ATP6V1A monoclonal antibody (M02), clone 4F5.Lane 1: ATP6V1A transfected lysate (Predicted MW: 68.3 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ATP6V1A Antibody (4F5) [H00000523-M02] - ATP6V1A monoclonal antibody (M02), clone 4F5. Analysis of ATP6V1A expression in human kidney.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ATP6V1A Antibody (4F5) [H00000523-M02] - Detection limit for recombinant GST tagged ATP6V1A is 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of monoclonal antibody to ATP6V1A on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ATP6V1A Antibody (4F5) [H00000523-M02] - Analysis of monoclonal antibody to ATP6V1A on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.
