- PA5-29191 - Invitrogen Antibodies ATP6V1A antibody

Submitted references Chronic activation of AMP-activated protein kinase leads to early-onset polycystic kidney phenotype.

Wilson L, Pollard AE, Penfold L, Muckett PJ, Whilding C, Bohlooly-Y M, Wilson P, Carling D
Clinical science (London, England : 1979) 2021 Oct 29;135(20):2393-2408

Systemic Blockade of Clever-1 Elicits Lymphocyte Activation Alongside Checkpoint Molecule Downregulation in Patients with Solid Tumors: Results from a Phase I/II Clinical Trial.

Virtakoivu R, Rannikko JH, Viitala M, Vaura F, Takeda A, Lönnberg T, Koivunen J, Jaakkola P, Pasanen A, Shetty S, de Jonge MJA, Robbrecht D, Ma YT, Skyttä T, Minchom A, Jalkanen S, Karvonen MK, Mandelin J, Bono P, Hollmén M
Clinical cancer research : an official journal of the American Association for Cancer Research 2021 Aug 1;27(15):4205-4220

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ATP6V1A using 30 µg of A) HeLa and B) HepG2 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an ATP6V1A polyclonal antibody (Product # PA5-29191) at a dilution of 1:10,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ATP6V1A was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ATP6V1A Polyclonal Antibody (Product # PA5-29191) at a dilution of 1:10000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ATP6V1A Polyclonal Antibody (Product # PA5-29191). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 7.5% SDS PAGE. ATP6V1A Polyclonal Antibody (Product # PA5-29191) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot was performed using Anti-ATP6V1A Polyclonal Antibody (Product # PA5-29191) and a 70 kDa band corresponding to V-type proton ATPase catalytic subunit A was observed across tissues tested. Whole cell extracts (30 µg lysate) of Mouse Brain (Lane 1), Rat Brain (Lane 2), Mouse Skeletal Muscle (Lane 3), Rat Skeletal Muscle (Lane 4), Mouse Heart (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 Dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of ATP6V1A in methanol-fixed A431 cells using an ATP6V1A polyclonal antibody (Product # PA5-29191) at a 1:500 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ATP6V1A Polyclonal Antibody detects ATP6V1A protein at cytoplasm on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. ATP6V1A Polyclonal Antibody (Product # PA5-29191) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PA5-29191

Antibody data