HPA031997

antibody from Atlas Antibodies

Targeting: HPDL 4-HPPD-L, GLOXD1, MGC15668

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA031997

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA031997, RRID:AB_10602909

Product name

Anti-HPDL

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human HPDL, Gene description: 4-hydroxyphenylpyruvate dioxygenase-like, Alternative Gene Names: 4-HPPD-L, GLOXD1, MGC15668, Validated applications: ICC, WB, Uniprot ID: Q96IR7, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.4 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell line CACO-2 and human cell line PC-3.

Orthogonal method

Orthogonal validation of protein expression using WB by comparison to RNA-seq data of corresponding target in high and low expression cell lines.

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Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Western blot analysis in human cell line CACO-2 and human cell line PC-3.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Appendix

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Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line CACO-2 shows localization to mitochondria.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line CACO-2 shows localization to mitochondria.

Sample type

Human

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubule.

Sample type

HUMAN