Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- EB07316 - Provider product page
- Provider
- Everest Biotech
- Proper citation
- Everest Biotech Cat#EB07316, RRID:AB_2294799
- Product name
- Goat anti-GPX4 (Isoform a and c) Antibody
- Antibody type
- Polyclonal
- Description
- Goat anti-GPX4 (Isoform a and c) Antibody
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-EEPLVIEKDLPHY
- Epitope
- C Terminus
- Isotype
- IgG
- Vial size
- 100µg specific antibody in 200µl
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
Submitted references Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans.
SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress.
Schoenmakers E, Agostini M, Mitchell C, Schoenmakers N, Papp L, Rajanayagam O, Padidela R, Ceron-Gutierrez L, Doffinger R, Prevosto C, Luan J, Montano S, Lu J, Castanet M, Clemons N, Groeneveld M, Castets P, Karbaschi M, Aitken S, Dixon A, Williams J, Campi I, Blount M, Burton H, Muntoni F, O'Donovan D, Dean A, Warren A, Brierley C, Baguley D, Guicheney P, Fitzgerald R, Coles A, Gaston H, Todd P, Holmgren A, Khanna KK, Cooke M, Semple R, Halsall D, Wareham N, Schwabe J, Grasso L, Beck-Peccoz P, Ogunko A, Dattani M, Gurnell M, Chatterjee K
The Journal of clinical investigation 2010 Dec;120(12):4220-35
The Journal of clinical investigation 2010 Dec;120(12):4220-35
SECIS-binding protein 2 promotes cell survival by protecting against oxidative stress.
Papp LV, Lu J, Bolderson E, Boucher D, Singh R, Holmgren A, Khanna KK
Antioxidants & redox signaling 2010 Apr 1;12(7):797-808
Antioxidants & redox signaling 2010 Apr 1;12(7):797-808
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Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB07316 (0.1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Everest Biotech (provider)
- Main image
- Experimental details
- EB07316 (0.3µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.