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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB5457 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat Glutathione Peroxidase 4/GPX4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects endogenous human, mouse and rat GPX4 in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P36969- Isotype
- IgG
- Antibody clone number
- 565320
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Targeting redox homeostasis in rhabdomyosarcoma cells: GSH-depleting agents enhance auranofin-induced cell death.
Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death.
Habermann KJ, Grünewald L, van Wijk S, Fulda S
Cell death & disease 2017 Oct 5;8(10):e3067
Cell death & disease 2017 Oct 5;8(10):e3067
Impairment of antioxidant defense via glutathione depletion sensitizes acute lymphoblastic leukemia cells for Smac mimetic-induced cell death.
Schoeneberger H, Belz K, Schenk B, Fulda S
Oncogene 2015 Jul 30;34(31):4032-43
Oncogene 2015 Jul 30;34(31):4032-43
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Detection of Human/Mouse/Rat Glutathione Peroxidase 4 by Western Blot. Western blot shows lysates of mouse, human, and rat liver tissue. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Glutathione Peroxidase 4 Monoclonal Antibody (Catalog # MAB5457) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Glutathione Peroxidase 4 at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
