Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AER-011-200UL - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EphA1 (extracellular) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Reconstitution: 1 X 50 µL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µL
- Concentration
- 0.8 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-EphA1 (extracellular) Antibody (#AER-011), (1:200).3,4. Anti-EphA1 (extracellular) Antibody , preincubated with EphA1 (extracellular) Blocking Peptide (#BLP-ER011).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human Jurkat T cell leukemia cells (lanes 1 and 5), human HeLa cervix adenocarcinoma cells (lanes 2 and 6), human SH-SY5Y neuroblastoma cells (lanes 3 and 7) and human MCF-7 breast adenocarcinoma cells (lanes 4 and 8): - 1-4. Anti-EphA1 (extracellular) Antibody (#AER-011), (1:200).5-8. Anti-EphA1 (extracellular) Antibody , preincubated with EphA1 (extracellular) Blocking Peptide (#BLP-ER011).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of EphA1 in human MCF-7 cells - Cell surface detection of EphA1 in live intact MCF-7 breast adenocarcinoma cells. Cells were stained with Anti-EphA1 (extracellular) Antibody (#AER-011), (1:25) followed by goat Anti-rabbit-AlexaFluor-594 secondary Antibody (red). B. Live image of the cells stained in A. C. Merge image of A and B.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Expression of EphA1 in rat striatum. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-EphA1 (extracellular) Antibody (#AER-011), (1:300), followed by donkey Anti-rabbit-biotin and Streptavidin-Cy3. A. EphA1 immunoreactivity (red) appears in striatal neurons (arrows). B. Pre-incubation of the Antibody with EphA1 (extracellular) Blocking Peptide (#BLP-ER011), suppressed staining. Cell nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Cell surface detection ofEphA1by indirect flow cytometry in live intactmouse J774 macrophage cells: - (black line) cells. (red) Cells+ goat- Anti-rabbit-FITC. (green) Cells + Anti-EphA1 (extracellular) Antibody (#AER-011), (2.5μg) + goat- Anti-rabbit-FITC).