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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 14732-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- hD53/TPD52L1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Four variants resulted from alternative splicing have been predicated and this antibody is expected to recognize all the variants.
- Concentration
- 0.21 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCF7 cells were subjected to SDS PAGE followed by western blot with 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of MCF-7 cells using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human prostate using 14732-1-AP (hD53; TPD52L1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-hD53; TPD52L1 (IP:14732-1-AP, 3ug; Detection:14732-1-AP 1:300) with MCF-7 cells lysate 2500ug.
