Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB11431 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11431, RRID:AB_1679721
- Product name
- LTF polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of LTF.
- Antigen sequence
C-ENYKSQQSSDPDP
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Lactoferrin acts as an alarmin to promote the recruitment and activation of APCs and antigen-specific immune responses.
de la Rosa G, Yang D, Tewary P, Varadhachary A, Oppenheim JJ
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6868-76
Journal of immunology (Baltimore, Md. : 1950) 2008 May 15;180(10):6868-76
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- LTF polyclonal antibody (Cat # PAB11431) (0.05 ug/mL) staining of human peripheral blood mononucleocyte (PBMCs) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- LTF polyclonal antibody (Cat # PAB11431) (5 ug/mL) as the reporter with the capture rabbit antibody (5 ug/mL).
- Validation comment
- Sandwich ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- LTF polyclonal antibody (Cat # PAB11431) (2 ug/mL) staining of paraffin embedded human blood vessel. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)