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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Immunohistochemistry [1]
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- Product number
- A00633-1 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Lactoferrin/LTF Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Rabbit IgG polyclonal antibody for Lactoferrin detection. Tested with WB, IHC-P, IHC-F, ICC, IF, FCM, Direct ELISA in Human;Mouse;Rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Identification and experimental validation of ferroptosis-related gene lactotransferrin in age-related hearing loss.
Zeng C, Gu X, Chen Y, Lin Y, Chen J, Chen Z, Chen C, Yao G, Lin C
Frontiers in aging neuroscience 2024;16:1309115
Frontiers in aging neuroscience 2024;16:1309115
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of Lactoferrin using anti-Lactoferrin antibody (A00633-1). Lactoferrin was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Lactoferrin Antibody (A00633-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
