MAB2310

antibody from R&D Systems

Targeting: RPS6KA4 MSK2, RSK-B

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2310

Provider

R&D Systems

Product name

Human/Mouse MSK2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse MSK2 in Western blots. Does not detect recombinant human MSK1.

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

O75676


Isotype

IgG

Antibody clone number

261034

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Mitogen- and stress-activated protein kinase 2 and cyclic AMP response element binding protein are activated in lesional psoriatic epidermis.

Funding AT, Johansen C, Kragballe K, Iversen L
The Journal of investigative dermatology 2007 Aug;127(8):2012-9

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse MSK2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse MSK2 Monoclonal Antibody (Catalog # MAB2310) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK2 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.