Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R31762 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- PRDX3 Antibody (Peroxiredoxin 3)
- Antibody type
- Polyclonal
- Antigen
- Human partial recombinant protein (AA 110-256) was used as the immunogen for this PRDX3 antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the PRDX3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of PRDX3 antibody and Lane 1: rat brain; 2: mouse brain; 3: rat skeletal muscle; 4: mouse skeletal muscle; 5: human U20S; 6: HeLa; 7: SMMC-7721; 8: RH35; 9: A549 lysate. Expected size ~28KD
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human HeLa cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human MCF7 cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of FFPE human SMMC-7721 cells with PRDX3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: PRDX3 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.