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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00010935-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00010935-M01, RRID:AB_547280
- Product name
- Mouse Monoclonal Peroxiredoxin 3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PRDX3 - peroxiredoxin 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references mTor is a signaling hub in cell survival: a mass-spectrometry-based proteomics investigation.
Tang Z, Baykal AT, Gao H, Quezada HC, Zhang H, Bereczki E, Serhatli M, Baykal B, Acioglu C, Wang S, Ioja E, Ji X, Zhang Y, Guan Z, Winblad B, Pei JJ
Journal of proteome research 2014 May 2;13(5):2433-44
Journal of proteome research 2014 May 2;13(5):2433-44
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of PRDX3 expression in transfected 293T cell line by PRDX3 monoclonal antibody (M01), clone 1E3-B2.Lane 1: PRDX3 transfected lysate (Predicted MW: 27.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Detection limit for recombinant GST tagged PRDX3 is approximately 1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of monoclonal antibody to PRDX3 on HeLa cell . Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Peroxiredoxin 3 Antibody (1E3-B2) [H00010935-M01] - Analysis of PRDX3 transfected lysate using anti-PRDX3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PRDX3 MaxPab rabbit polyclonal antibody.
