MA5-32721

antibody from Invitrogen Antibodies

Targeting: PRDX3 AOP-1, AOP1, MER5, SP-22

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-32721

Provider

Invitrogen Antibodies

Product name

PRDX3 Recombinant Rabbit Monoclonal Antibody (JA53-21)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JA53-21

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PRDX3 in different lysates using a Monoclonal antibody (Product #MA5-32721) at a dilution of 1:1,000. Positive control: Lane1: Human liver, Lane 2: MCF-7, Lane 3: A431.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of PRDX3 in Hela cells using a PRDX3 Monoclonal antibody (Product # MA5-32721) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of PRDX3 in HepG2 cells using a PRDX3 Monoclonal antibody (Product # MA5-32721) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of PRDX3 in MCF-7 cells using a PRDX3 Monoclonal antibody (Product # MA5-32721) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemical analysis of PRDX3 in MCF-7 cells using a PRDX3 Monoclonal antibody (Product # MA5-32721) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PRDX3 of paraffin-embedded Human liver tissue using a PRDX3 Monoclonal antibody (Product #MA5-32721). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PRDX3 of paraffin-embedded Human liver cancer tissue using a PRDX3 Monoclonal antibody (Product #MA5-32721). Counter stained with hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of PRDX3 of paraffin-embedded Human kidney tissue using a PRDX3 Monoclonal antibody (Product #MA5-32721). Counter stained with hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of PRDX3 in MCF-7 cells using a PRDX3 Monoclonal Antibody (Product # MA5-32721) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometric analysis of PRDX3 in MCF-7 cells using a PRDX3 Monoclonal Antibody (Product # MA5-32721) at a dilution of 1:100, as seen in red compared with an unlabelled control (cells without incubation with primary antibody; black).