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LearnPA5-21027
antibody from Invitrogen Antibodies
Targeting: NLRP12
CLR19.3, Monarch1, NALP12, PAN6, PYPAF7, RNO2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [1]
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- Product number
- PA5-21027 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NALP12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is human brain tissue lysate. PA5-21027 can be used with blocking peptide PEP-1141.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species.
Moustaqil M, Ollivier E, Chiu HP, Van Tol S, Rudolffi-Soto P, Stevens C, Bhumkar A, Hunter DJB, Freiberg AN, Jacques D, Lee B, Sierecki E, Gambin Y
Emerging microbes & infections 2021 Dec;10(1):178-195
Emerging microbes & infections 2021 Dec;10(1):178-195
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human brain tissue lysate using a NALP12 polyclonal antibody (Product # PA5-21027) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of NALP12 in human brain tissue lysate with NALP12 Polyclonal Antibody (Product # PA5-21027) at 1 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of NALP12 in human brain tissue with NALP12 Polyclonal Antibody (Product # PA5-21027) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of NALP12 in human brain tissue with NALP12 Polyclonal Antibody (Product # PA5-21027) at 20 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of NALP12 in human brain tissue with NALP12 Polyclonal Antibody (Product # PA5-21027) at 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5. IRF3, TAB1 and NLRP12 are decreased upon infection with SARS-CoV-2 in 293T-ACE2 cells, in two independent laboratories. (A-C) 293T-ACE2 cells were infected with SARS-CoV-2 and analysed for viral and host protein levels 72 h post-infection. (D-I) Independently, stable 293T-ACE2 were infected with icSARS-CoV-2 mNeonGreen) and the levels of IRF3, TAB1 And NLRP12 were visualized by Western Blotting at 6 h (D), 24 h (E) and 48 h (F). (G-I): Protein levels measured by densitometry for IRF3 (G), TAB1 (H) and NLRP12 (I) and plotted for the Mock, poly(I:C) and SARS-CoV-2 infection at 6 h, 24 h and 48 h.
