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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 66314-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RSPO3 Monoclonal Antibody (1E4F1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: GRRQRRMHP NVSQGCQGGC ATCSDYNGCL SCKPRLFFAL ERIGMKQIGV CLSSCPSGYY GTRYPDINKC TKCKADCDTC FNKNFCTKCK SGFYLHLGKC LDNCPEGLEA NNHTMECVSI VHCEVSEWNP WSPCTKKGKT CGFKRGTETR VREIIQHPSA KGNLCPPTNE TRKCTVQRKK CQKGERGKKG RERKRKKPNK GESKEAIPDS KSLESSKEIP EQRENKQQQK KRKVQDKQKS VSVSTVH (27-272 aa encoded by BC022367)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E4F1
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66314-1-IG ( RSPO3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A549 cells were subjected to SDS PAGE followed by western blot with 66314-1-Ig (RSPO3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed A549 cells using 66314-1-IG (RSPO3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66314-1-IG ( RSPO3 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66314-1-IG ( RSPO3 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
