- PA3-753 - Invitrogen Antibodies PRDX4 antibody

Submitted references Overexpression of PRDX4 Modulates Tumor Microenvironment and Promotes Urethane-Induced Lung Tumorigenesis.

Zheng J, Guo X, Nakamura Y, Zhou X, Yamaguchi R, Zhang J, Ishigaki Y, Uramoto H, Yamada S
Oxidative medicine and cellular longevity 2020;2020:8262730

Peroxiredoxin 4 promotes embryonal hepatoblastoma cell migration but induces fetal cell differentiation.

Zheng J, Guo X, Shioya A, Yoshioka T, Matsumoto K, Hiraki T, Kusano H, Oyama T, Kurose N, Yamaguchi R, Uramoto H, Ieiri S, Okajima H, Kohno M, Yamada S
American journal of translational research 2020;12(6):2726-2737

Quantitative Proteomics Reveal Peroxiredoxin Perturbation Upon Persistent Lymphocytic Choriomeningitis Virus Infection in Human Cells.

Benej M, Danchenko M, Oveckova I, Cervenak F, Tomaska L, Grossmannova K, Polcicova K, Golias T, Tomaskova J
Frontiers in microbiology 2019;10:2438

Nonredundant antioxidant defense by multiple two-cysteine peroxiredoxins in human prostate cancer cells.

Shen C, Nathan C
Molecular medicine (Cambridge, Mass.) 2002 Feb;8(2):95-102

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), DU-145 (Lane 2), PC-3 (Lane 3), LNCaP (Lane 4), MDA-MB-231 (Lane 5), Hep G2 (Lane 6), A549 (Lane 7) and U-937 (Lane 8). The blot was probed with Anti-Peroxiredoxin 4 Rabbit Polyclonal Antibody (Product # PA3-753, 1:5000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). Doublet bands observed around 22 kDa, across the cell lines, correspond to Peroxiredoxin 4. Known quantity of protein samples were electrophoresed using Novex® NuPAGE&reg ; 12% Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of Peroxiredoxin 4 was performed using 70% confluent log phase LNCaP cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRDX4 Rabbit Polyclonal Antibody (Product # PA3-753) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunocytochemical staining of Prx 4 in TSU-Pr1 cells using Product # PA3-753.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 1 Elevated expressions of PRDX4 in Tg mice after urethane stimulation. (a) Comparisons of PRDX4 expression between Tg and non-Tg controls in initial lung tissues, in lung tissues after urethane stimulation, and in lung tumor tissues, respectively, by Western blotting. (b) Serum hPRDX4 expressions detected by the ELISA method in the process of tumor formation. The values represent mean +- SD, and the experiments shown were repeated in triplicate with similar results.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 5 ROS production in LCMV- and mock-infected HeLa cells. (A) Immunoblot analysis of PRDX4 (left) with specific antibody using whole-cell extracts prepared from LCMV-infected HeLa cells (HeLa-MX) and mock-infected HeLa cells (HeLa). The signal obtained with anti-beta-actin antibody was used as loading control. One representative of three biological replicates is shown. Densitometric analysis of protein abundance (right) was performed using ImageJ software. Relative quantity of proteins was calculated as the ratio of the intensity of each signal to the intensity of the related beta-actin internal standard. Values represent the means of three biological replicates. Error bars denote standard deviations. ** P < 0.01 (HeLa-MX vs. HeLa). (B) ROS generation was assessed in a microplate reader using DCF fluorescence and normalized to the number of cells measured by DAPI staining. The results represent the mean from three independent biological experiments, each done in eight replicates. Error bars denote standard deviations. Data are presented as relative increase compared to control (HeLa cells), which was set to 1. **** P < 0.0001 (HeLa-MX vs. HeLa).

PA3-753

Antibody data