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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB4539 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4539, RRID:AB_1579141
- Product name
- PRKCG polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PRKCG.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC.
Spinocerebellar ataxia type 14 caused by a mutation in protein kinase C gamma.
Identification of a novel SCA14 mutation in a Dutch autosomal dominant cerebellar ataxia family.
Uchino M, Sakai N, Kashiwagi K, Shirai Y, Shinohara Y, Hirose K, Iino M, Yamamura T, Saito N
The Journal of biological chemistry 2004 Jan 16;279(3):2254-61
The Journal of biological chemistry 2004 Jan 16;279(3):2254-61
Spinocerebellar ataxia type 14 caused by a mutation in protein kinase C gamma.
Yabe I, Sasaki H, Chen DH, Raskind WH, Bird TD, Yamashita I, Tsuji S, Kikuchi S, Tashiro K
Archives of neurology 2003 Dec;60(12):1749-51
Archives of neurology 2003 Dec;60(12):1749-51
Identification of a novel SCA14 mutation in a Dutch autosomal dominant cerebellar ataxia family.
van de Warrenburg BP, Verbeek DS, Piersma SJ, Hennekam FA, Pearson PL, Knoers NV, Kremer HP, Sinke RJ
Neurology 2003 Dec 23;61(12):1760-5
Neurology 2003 Dec 23;61(12):1760-5
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PRKCG polyclonal antibody (Cat # PAB4539) in mouse brain lysate . PRKCG (arrow) was detected using purified PRKCG polyclonal antibody (Cat # PAB4539) . Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human brain tissue reacted with PRKCG polyclonal antibody (Cat # PAB4539) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining . This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated .
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
