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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- 66429-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PKC gamma Monoclonal Antibody (2F4B9)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: FSFLMVLGK GSFGKVMLAE RRGSDELYAI KILKKDVIVQ DDDVDCTLVE KRVLALGGRG PGGRPHFLTQ LHSTFQTPDR LYFVMEYVTG GDLMYHIQQL GKFKEPHAAF YAAEIAIGLF FLHNQGIIYR DLKLDNVMLD AEGHIKITDF GMCKENVFPG TTTRTFCGTP DYIAPEIIAY QPYGKSVDWW SFGVLLYEML AGQPPFDGED EEELFQAIME QTVTYPKSLS REAVAICKGF LTKHPGKRLG SGPDGEPTIR AHGFFRWIDW ERLERLEIPP PFRPRPCGRS GENFDKFFTR AAPALTPPDR LVLASIDQAD FQGFTYVNPD FVHPDARSPT SPVPVPVM (351-697 aa encoded by BC047876)
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2F4B9
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Various lysates were subjected to SDS PAGE followed by western blot with 66429-1-IG ( PKC gamma antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 66429-1-IG (PKC gamma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 66429-1-IG (PKC gamma antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 66429-1-IG ( PKC gamma antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human brain tissue slide using 66429-1-IG ( PKC gamma antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 SH-SY5Y cells were intracellularly stained with 0.2 µg Anti-Human PKC Gamma (Product # 66429-1-IG, Clone:2F4B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
