Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AM1836A - Provider product page
- Provider
- Abgent
- Proper citation
- Abgent Cat#AM1836a, RRID:AB_10664786
- Product name
- EZH2 Antibody
- Antibody type
- Monoclonal
- Antigen
- Recombinant Protein
- Description
- Purified Mouse Monoclonal Antibody (Mab)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.5 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- Western blot analysis of EZH2 antibody (Cat. #AM1836a) in T47D cell line lysates (35ug/lane).EZH2 (arrow) was detected using the purified Mab.
- Primary Ab dilution
- 1:100~500
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "Fluorescent image of U251 cell stained with EZH2 Antibody(Cat#AM1836a/SG100830AA).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EZH2 primary antibody (1:25, 1 h at 37!). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37!).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37!).EZH2 immunoreactivity is localized to Nucleus significantly."
- Primary Ab dilution
- 1:10~50
Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- EZH2 Monoclonal Antibody (Cat. #AM1836a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EZH2 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
- Primary Ab dilution
- 1:10~50
