GTX60811

antibody from GeneTex

Targeting: EZH2 ENX-1, EZH1, KMT6, KMT6A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX60811

Provider

GeneTex

Product name

EZH2 antibody - ChIP grade

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of nuclear extracts from HeLa cells using EZH2 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

WB analysis of nuclear extracts from HeLa cells using EZH2 antibody at a dilution of 1:1,000 in TBS-Tween containing 5% skimmed milk.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ChIP was performed with HeLa extracts with control IgG (2 ?g)and EZH2 antibody (0.5, 1, 2.5 and 5 ?g per ChIP experiment). The precipitated DNA was detected by qPCR with primers targeting to MYT1 and GAPDH promoters.

Validation comment

ChIP

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

ChIP assays were performed using K562 cells, the GeneTex antibody against EZH2 (Cat. No. GTX60811) and optimized PCR primer sets for qPCR. ChIP was performed with the ¡§iDeal ChIP-seq¡¨ kit using sheared chromatin from 4 million cells. A titration of the antibody consisting of 1, 2. 5 and 10 ?g per ChIP experiment was analysed. IgG (2 ?g/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls. Figure 1 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).